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You are here: Home > Hungate Tubes

Hungate Type Anaerobic Culture Tube

Gas tight tube designed for obtaining and maintaining anaerobic culture conditions
Method of Hungate et.al, utilizes syringe methods for anaerobiosis
Anaerobic culture tubes contain three autoclavable parts; a screw cap with a 9mm opening, a flange type non toxic butyl rubber stopper that is gas impermeable, and a disposable screw cap culture tube

Special Notes

Hungate Culture Tube Technique
The Hungate Anaerobic Culture Tube was developed for growing and maintaining anaerobic bacteria and culture conditions. The technique is simple:
Exclude oxygen by flushing the tube with the desired gas
Place 4.5ml of pre-reduced anaerobic agar medium into tube
Seal the tube with the butyl rubber stopper and screw cap
Autoclave the tube
Inoculate with a syringe
Prepare on roll tube spinner
Incubate in water bath

References:Macy, J.M., Snellen, J.E. and R.E. Hungate. 1972. Use of Syringe Methods for Anaerobiosis.. Clin. Nutr. December.R.E. Hungate. " A Roll Tube Method For Cultivation of Strict Anaerobes" in Methods of Microbiology, Vol. 111pps 117-132, Academic Press, London. The Rumen And Its Microbes", R.E. Hungate, 533pp(1966), Academic Press, N.Y